| Acronym | Definition |
|---|
| SPOT | Screening of Passengers by Observation Techniques |
| SPOT | Searching Peak of Tension (polygraph testing) |
| SPOT | Sensor Placement Optimization Tool (drinking water research) |
| SPOT | Simply Protecting Our Tots |
| SPOT | Swiss Professional Officer Training Course |
| SPOT | Synchronized Pre-Deployment and Operational Tracker (bar-code system used to track travel/movement of US DoD contractors) |
| SPOT | Synchronized Predeployment and Operational Tracker (US Department of Defense program for tracking deployed contractors) |