| Acronym | Meaning |
| DFO | Date File Opened (US DHS) |
| DFO | Department of Fisheries and Oceans (Canada) |
| DFO | Designated Federal Official |
| DFO | Diesel Fuel Oil |
| DFO | Dining Facility Operation |
| DFO | Director of Field Operations |
| DFO | Disaster Field Office (US FEMA) |
| DFO | Distance from Origin |
| DFO | Distillate Fuel Oil |
| DFO | District Forest Office |
| DFO | Divisional Finance Officer |
| DFO | Divisional Forest Officer |
| DFO | Donor Funded Operation (World Bank) |
| DFO | Duty Field Officer (UK) |